Environmental Stresses on Spore Populations of Bacillus stearothermophilus

Environmental Stresses on Spore Populations of Bacillus Stearothermophilus.

Heat-shocking spores at 110 C in 20% sucrose solutions decreased the percentage of the rough variant in a mixed population (rough and smooth variants) of strain M. Heat-shocking spores of the rough variant of strain NCA 1518 in 20% sucrose produced a decline in the number which germinated, whereas the smooth variant of strain NCA 1518 increased in the number which germinated. By the use of phas...

Spore production by Bacillus stearothermophilus.

Methods reported in the literature for the production of spores by Bacillus stearothermophilus require from 4 to 14 days of incubation (N. Finley and M. L. Fields, Appl. Microbiol. 10:231, 1962; D. I-C. Wang, J. Scharer, and A. E. Humphrey, Appl. Microbiol. 12:451, 1964; L. L. Campbell, C. M. Richards, and E. E. Sniff, p. 55, in L. L. Campbell and H. 0. Halvorson [ed.], Spores III, American Soc...

Comparative characterization of silver nanoparticles synthesized by spore extract of Bacillus subtilis and Geobacillus stearothermophilus

Objective(s): Silver nanostructures have gathered remarkable attention due to their applications in diversefields. Researchers have recently demonstrated that bacterial spores are capable of reducing silver ions toelemental silver leading to formation of nanoparticles.Materials and Methods: In this study, spores of Bacillus subtilis and Geobacillus stearothermophilus wereemployed to produce sil...

Sporulation of Bacillus stearothermophilus.

A broth medium containing tryptone and manganese sulfate supported heavy sporulation of Bacillus stearothermophilus ATCC 7953 (NCA 1518) and four isolates identified as B. stearothermophilus. Maximal spore yields were obtained by use of inocula grown anaerobically in a medium containing glucose with aeration of sporulation medium via bubbling. After an extended stationary period, sporulation oc...

Thermocins of Bacillus stearothermophilus.